Ranomics
Scientist operating automated laboratory equipment for high-throughput protein screening
Packaged program

AI Binder Sprint

A fixed-scope, 6-8 week protein binder discovery program delivering experimentally validated binders from your target structure

RFdiffusionBindCraftBoltzgenYeast Display
Who this is for

Built for biotech teams that need binders fast

R&D teams building a binder pipeline

Companies validating a target or building a pipeline that needs a binder to advance into IND-enabling studies, secure a partnership, or de-risk a program.

Teams without display infrastructure

Organizations that do not have in-house yeast display, FACS sorting, or NGS sequencing capabilities but need the experimental validation that only display provides.

Timeline-constrained programs

Projects with a defined target and a hard deadline: conference readout, partnership data package, or a funding milestone that depends on having binding data in hand.

Novel or difficult targets

Targets where conventional library approaches have failed or where no existing binder scaffold provides a starting point. De novo design is strongest exactly here — including intracellular targets, which we screen by fusing to membrane anchors on our display platform.

IP and freedom-to-operate considerations

De novo designed binders are novel sequences with no derivation from existing antibody germlines or proprietary libraries. This simplifies IP strategy and freedom-to-operate analysis compared to binders derived from immunization or licensed scaffold platforms.

Scope

What's included in the AI Binder Sprint

Included

Hotspot analysis of your provided target structure
RFdiffusion, BindCraft, and Boltzgen campaigns (500-2,000 candidates)
In silico filtering and structural validation
Gene synthesis of filtered candidate pool
Yeast or mammalian surface display
One round of FACS/MACS selection
NGS sequencing and hit analysis
Final report with ranked binder hits, sequence files, and binding evidence

Not included (available as add-ons)

Downstream expression scale-up
SPR/ITC affinity characterization
Additional FACS selection rounds
Affinity maturation campaigns
Timeline

How the binder sprint runs

Three phases over 6-8 weeks. Milestone check-ins at the end of each phase.

1
Week 1-2

Design

Target structure intake, hotspot definition, RFdiffusion / BindCraft / Boltzgen campaigns, in silico filtering and structural validation.

2
Week 3-5

Build and screen

Gene synthesis, cloning into display construct, yeast or mammalian display preparation, selection rounds (FACS or MACS).

3
Week 6-8

Analyze and deliver

NGS sequencing of sorted populations, hit calling and enrichment analysis, ranked hit list, final report delivery.

Your inputs

What you provide to start the sprint

The sprint is designed to minimize demands on your team. Here is what we need from you to start.

Target structure (PDB, AlphaFold model, or equivalent confidence)
Defined binding region or functional notes on the epitope of interest
Signed SOW and mutual NDA
Approximately 2 hours total for kickoff call and milestone check-ins
FAQ

Sprint questions

Can I run a sprint on a target with no existing structure? +

We can work with high-confidence AlphaFold2 or AF3 models. Targets with no structural model are assessed case-by-case. Contact us to discuss feasibility before applying.

What is the expected hit rate? +

Validated hit rates vary by target difficulty, epitope accessibility, and scaffold format. Pilot data on soluble targets have yielded 2-12 confirmed binders per 1,000 screened candidates. We assess feasibility on a per-target basis before scoping the sprint.

Can I extend the sprint with additional selection rounds? +

Yes. Additional FACS rounds, affinity maturation campaigns, and downstream characterization (SPR, BLI) are available as extensions to the base sprint scope. These are scoped separately after the first round of hits is identified.

Is a sprint appropriate for intracellular targets? +

Yes. Intracellular targets are screened by fusing the target protein to a membrane anchor on our display platform, presenting it on the cell surface for selection. This allows the same FACS-based workflow used for extracellular targets. Contact us to discuss your specific target and display strategy.

How are project results kept confidential? +

All projects run under mutual NDA. Target structures, designed sequences, and screening data are not shared or used outside your project. We do not retain client sequence data beyond project delivery.

Spots are limited. Projects start on a rolling basis.

Apply now to discuss your target and confirm availability. We will assess feasibility and send you a proposed SOW within 5 business days.